A gRNA molecule of claim 4, wherein the targeting domain comprises, e.g., consists of, SEQ ID NO: 338.ДЎ0. A gRNA molecule of claim 4, wherein the targeting domain comprises, e.g., consists of, SEQ ID NO: 248.Д©.

A gRNA molecule of claim 4, wherein the targeting domain comprises, e.g., consists of, any one of SEQ ID NO: 248 or SEQ ID NO: 338.ДЁ. A gRNA molecule of claim 4, wherein the targeting domain comprises, e.g., consists of, any one of SEQ ID NO: 247, SEQ ID NO: 248, SEQ ID NO: 335, SEQ ID NO: 336, SEQ ID NO: 337, or SEQ ID NO: 338.Д§. A gRNA molecule of claim 4, wherein the targeting domain comprises, e.g., consists of, any one of SEQ ID NO: 341, SEQ ID NO: 246, SEQ ID NO: 248, SEQ ID NO: 247, SEQ ID NO: 245, SEQ ID NO: 249, SEQ ID NO: 244, SEQ ID NO: 199, SEQ ID NO: 251, SEQ ID NO: 250, SEQ ID NO: 334, SEQ ID NO: 185, SEQ ID NO: 186, SEQ ID NO: 336, or SEQ ID NO: 337.Д¦. A gRNA molecule of claim 1, wherein the target sequence is of a BCLl la enhancer, and the targeting domain comprises, e.g., consists of, any one of SEQ ID NO: 182 to SEQ ID NO: 277 or SEQ ID NO: 334 to SEQ ID NO: 341.ДҐ. A gRNA molecule of claim 1, wherein the target sequence is of a BCLl la enhancer, and the targeting domain comprises, e.g., consists of, any one of SEQ ID NO: 1232 to SEQ ID NO: 1499.Д¤. A gRNA molecule of claim 1, wherein the target sequence is of the BCLl 1A gene, and the targeting domain comprises, e.g., consists of, any one of SEQ ID NO: 1 to SEQ ID NO: 85 or SEQ ID NO: 400 to SEQ ID NO: 1231.ДЈ. A gRNA molecule comprising a tracr and crRNA, wherein the crRNA comprises a targeting domain that is complementary with a target sequence of a BCLl 1A gene (e.g., a human BCLl la gene), a BCLl la enhancer (e.g., a human BCLl la enhancer), or a HFPH region (e.g., a human HPFH region).Дў. SAMBROOK ET AL.: 'MOLECULAR CLONING: A LABORATORY MANUAL', vol. SAKSUMA ET AL.: 'MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems', NATURE PROTOCOLS, vol. MAKAROVA ET AL., NATURE REVIEW MICROBIOLOGY, vol. SLAYMAKER ET AL., SCIENCE EXPRESS, 1 December 2015 ()

337, 2012, pages 816Д¬HYLINSKI ET AL., RNA BIOLOGY, vol. SANKARAN VG ET AL.: 'A functional element necessary for fetal hemoglobin silencing', NEJM, vol.

WANG ET AL: 'Genetic Screens in Human Cells Using the CRISPR-Cas9 System', SCIENCE, vol. CANVER ET AL: 'BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis', NATURE, vol.

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